Journal: Cancers

Article Title: High CD73 Expression Is Associated with Poor Prognosis in Biliary Tract Cancer Through Reduced Stromal Tumor-Infiltrating Lymphocytes

doi: 10.3390/cancers18060975

Figure Lengend Snippet: Schematic illustration of the T-score and S-score. The tumor bed was divided into tumor and stromal compartments, and the T-score and S-score represent the quantitative measurements of CD73 (CD3, CD8, Foxp3, and CD163) expression or lymphocyte infiltration within each compartment, respectively.

Article Snippet: Eligible specimens were subjected to IHC staining using a rabbit monoclonal anti-CD73 antibody (#13160, Cell Signaling Technology, Danvers, MA, USA) diluted 1:100, a rabbit polyclonal anti-CD3 antibody (Dako #IR503, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD8 antibody (Dako #IR623, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD163 antibody (#CD163-L-CE, Leica Biosystems, Nussloch, Germany) diluted 1:500, and a mouse monoclonal anti-Foxp3 antibody (#ab20034, abcam, Cambridge, UK) diluted 1:100.

Techniques: Expressing

Journal: Cancers

Article Title: High CD73 Expression Is Associated with Poor Prognosis in Biliary Tract Cancer Through Reduced Stromal Tumor-Infiltrating Lymphocytes

doi: 10.3390/cancers18060975

Figure Lengend Snippet: Representative IHC staining images ( left ) and corresponding AI-based analysis images generated by DeepPathFinder™ ( right ) for CD73, CD3, CD8, Foxp3, and CD163 (original magnification approximately ×200). IHC-positive areas (cyan) were automatically identified and overlaid onto tumor regions (green) delineated on the corresponding H&E–stained images, enabling quantitative assessment of IHC-positive area within the tumor compartment.

Article Snippet: Eligible specimens were subjected to IHC staining using a rabbit monoclonal anti-CD73 antibody (#13160, Cell Signaling Technology, Danvers, MA, USA) diluted 1:100, a rabbit polyclonal anti-CD3 antibody (Dako #IR503, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD8 antibody (Dako #IR623, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD163 antibody (#CD163-L-CE, Leica Biosystems, Nussloch, Germany) diluted 1:500, and a mouse monoclonal anti-Foxp3 antibody (#ab20034, abcam, Cambridge, UK) diluted 1:100.

Techniques: Immunohistochemistry, Generated, Staining

Journal: Cancers

Article Title: High CD73 Expression Is Associated with Poor Prognosis in Biliary Tract Cancer Through Reduced Stromal Tumor-Infiltrating Lymphocytes

doi: 10.3390/cancers18060975

Figure Lengend Snippet: Effect of the CD73 and TIL scores. Kaplan–Meier curves of the overall survival in 100 patients ( a ) 50 in the T-CD73-high group and 50 in the T-CD73-low group ( b ) 50 in the S-CD73-high group and 50 in the S-CD73-low group ( c ) 50 in the T-TIL-high group and 50 in the T-TIL-low group ( d ) 50 in the S-TIL-high group and 50 in the S-TIL-low group.

Article Snippet: Eligible specimens were subjected to IHC staining using a rabbit monoclonal anti-CD73 antibody (#13160, Cell Signaling Technology, Danvers, MA, USA) diluted 1:100, a rabbit polyclonal anti-CD3 antibody (Dako #IR503, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD8 antibody (Dako #IR623, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD163 antibody (#CD163-L-CE, Leica Biosystems, Nussloch, Germany) diluted 1:500, and a mouse monoclonal anti-Foxp3 antibody (#ab20034, abcam, Cambridge, UK) diluted 1:100.

Techniques:

Journal: Cancers

Article Title: High CD73 Expression Is Associated with Poor Prognosis in Biliary Tract Cancer Through Reduced Stromal Tumor-Infiltrating Lymphocytes

doi: 10.3390/cancers18060975

Figure Lengend Snippet: Correlations between T-CD73 and TIL scores/immune cell subsets. Spearman’s rank correlation coefficient between ( a ) T-CD73 and T-TIL scores and ( b ) T-CD73 and S-TIL scores.

Article Snippet: Eligible specimens were subjected to IHC staining using a rabbit monoclonal anti-CD73 antibody (#13160, Cell Signaling Technology, Danvers, MA, USA) diluted 1:100, a rabbit polyclonal anti-CD3 antibody (Dako #IR503, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD8 antibody (Dako #IR623, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD163 antibody (#CD163-L-CE, Leica Biosystems, Nussloch, Germany) diluted 1:500, and a mouse monoclonal anti-Foxp3 antibody (#ab20034, abcam, Cambridge, UK) diluted 1:100.

Techniques:

Journal: Cancers

Article Title: High CD73 Expression Is Associated with Poor Prognosis in Biliary Tract Cancer Through Reduced Stromal Tumor-Infiltrating Lymphocytes

doi: 10.3390/cancers18060975

Figure Lengend Snippet: Correlations between T-CD73 and immune cell subsets. Spearman’s rank correlation coefficient between ( a ) T-CD73 and S-CD3 scores and ( b ) T-CD73 and S-CD8 scores.

Article Snippet: Eligible specimens were subjected to IHC staining using a rabbit monoclonal anti-CD73 antibody (#13160, Cell Signaling Technology, Danvers, MA, USA) diluted 1:100, a rabbit polyclonal anti-CD3 antibody (Dako #IR503, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD8 antibody (Dako #IR623, Agilent, Santa Clara, CA, USA), a mouse monoclonal anti-CD163 antibody (#CD163-L-CE, Leica Biosystems, Nussloch, Germany) diluted 1:500, and a mouse monoclonal anti-Foxp3 antibody (#ab20034, abcam, Cambridge, UK) diluted 1:100.

Techniques: